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Use of Rat Primary Mesenteric Cells for the Prediction of PDE4 Inhibitor Drug-Induced Vascular Injury.
Gosink, Mark M; Chapin, Robert E; Wilkie, Dean; Davenport, Scott; Kumpf, Steven W; Enerson, Bradley E; Houle, Christopher; Koza-Taylor, Petra; Wisialowski, Todd A; Lawton, Michael P.
Afiliação
  • Gosink MM; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Chapin RE; Preston, Connecticut 06365.
  • Wilkie D; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Davenport S; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Kumpf SW; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Enerson BE; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Houle C; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Koza-Taylor P; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Wisialowski TA; Drug Safety Research and Development, Pfizer Inc., Groton, Connecticut 06340.
  • Lawton MP; Westbrook, Connecticut 06498.
Toxicol Sci ; 159(1): 42-49, 2017 09 01.
Article em En | MEDLINE | ID: mdl-28903491
ABSTRACT
Drug-induced vascular injury (DIVI) in preclinical studies can delay, if not terminate, a drug development program. Clinical detection of DIVI can be very difficult as there are no definitive biomarkers known to reliably detect this disorder in all instances. The preclinical identification of DIVI requires detailed microscopic examination of a wide range of tissues although one of the most commonly affected areas in rats is the mesenteric vasculature. The reason for this predisposition of mesenteric arteries in rats as well as the exact mechanism and cell types involved in the initial development of these lesions have not been fully elucidated. We hypothesized that by using a mixed culture of cells from rat mesenteric tissue, we would be able to identify an RNA expression signature that could predict the invivo development of DIVI. Five compounds designed to inhibit Phosphodiesterase 4 activity (PDE4i) were chosen as positive controls. PDE4i's are well known to induce DIVI in the mesenteric vasculature of rats and there is microscopic evidence that this is associated, at least in part, with a proinflammatory mechanism. We surveyed, by qRT-PCR, the expression of 96 genes known to be involved in inflammation and using a Random-Forest model, identified 12 genes predictive of invivo DIVI outcomes in rats. Using these genes, we were able to cross-validate the ability of the Random-Forest modeling to predict the concentration at which PDE4i caused DIVI invivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões do Sistema Vascular / Inibidores da Fosfodiesterase 4 / Artérias Mesentéricas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões do Sistema Vascular / Inibidores da Fosfodiesterase 4 / Artérias Mesentéricas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article