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Interstitial telomeric repeats-associated DNA breaks.
Shubernetskaya, Olga; Skvortsov, Dmitry; Evfratov, Sergey; Rubtsova, Maria; Belova, Elena; Strelkova, Olga; Cherepaninets, Varvara; Zhironkina, Oxana; Olovnikov, Alexey; Zvereva, Maria; Dontsova, Olga; Kireev, Igor.
Afiliação
  • Shubernetskaya O; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Skvortsov D; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Evfratov S; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Rubtsova M; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Belova E; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Strelkova O; b A.N. Belozersky Institute of Physico-chemical Biology , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Cherepaninets V; b A.N. Belozersky Institute of Physico-chemical Biology , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Zhironkina O; b A.N. Belozersky Institute of Physico-chemical Biology , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Olovnikov A; c Institute of Biochemical Physics, RAS , Moscow , Russia.
  • Zvereva M; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Dontsova O; b A.N. Belozersky Institute of Physico-chemical Biology , M.V. Lomonosov Moscow State University , Moscow , Russia.
  • Kireev I; a Chemistry Department , M.V. Lomonosov Moscow State University , Moscow , Russia.
Nucleus ; 8(6): 641-653, 2017 11 02.
Article em En | MEDLINE | ID: mdl-28914588
During a cell's lifespan, DNA break formation is a common event, associated with many processes, from replication to apoptosis. Most of DNA breaks are readily repaired, but some are meant to persist in time, such as the chromosome ends, protected by telomeres. Besides them, eukaryotic genomes comprise shorter stretches of interstitial telomeric repeats. We assumed that the latter may also be associated with the formation of DNA breaks meant to persist in time. In zebrafish and mouse embryos, cells containing numerous breakage foci were identified. These breaks were not associated with apoptosis or replication, nor did they seem to activate DNA damage response machinery. Unlike short-living, accidental sparse breaks, the ones we found seem to be closely associated, forming discrete break foci. A PCR-based method was developed, allowing specific amplification of DNA regions located between inverted telomeric repeats associated with breaks. The cloning and sequencing of such DNA fragments were found to denote some specificity in their distribution for different tissue types and development stages.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sequências Repetitivas de Ácido Nucleico / Telômero / Quebras de DNA de Cadeia Dupla Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sequências Repetitivas de Ácido Nucleico / Telômero / Quebras de DNA de Cadeia Dupla Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article