A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species.
J Clin Microbiol
; 55(12): 3405-3410, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28954898
ABSTRACT
The carbapenem inactivation method (CIM) and modified CIM (mCIM) are simple and economical phenotypic screening methods for detecting carbapenemase production in Gram-negative bacteria. Although the mCIM has been recommended by the Clinical and Laboratory Standards Institute, both the CIM and mCIM have limitations. This study describes another modified CIM, called CIMTris, in which carbapenemase was extracted from bacteria with 0.5 M Tris-HCl (pH 7.6) buffer. The ability of the CIMTris to detect carbapenemase production was examined in Acinetobacter and Pseudomonas species. The CIMTris had an overall sensitivity of 97.6% and an overall specificity of 92.6%, whereas the mCIM had a sensitivity of 45.1% and a specificity of 100% for the isolates tested. These findings indicate that the CIMTris is useful for detecting carbapenemase production in Acinetobacter and Pseudomonas species.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas
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Proteínas de Bactérias
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Acinetobacter
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Beta-Lactamases
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Técnicas Bacteriológicas
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Testes Diagnósticos de Rotina
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Guideline
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article