Proteolytic activity assayed by subcellular localization switching of a substrate.
Biochem Biophys Rep
; 8: 23-28, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-28955937
ABSTRACT
An approach to assay proteolytic activity in vivo by altering the subcellular localization of a labelled substrate was demonstrated. The assay included a protein shuttling between different cellular compartments and a site-specific recombinant protease. The shuttle protein used was the human immunodeficiency virus type 1 (HIV-1) Rev protein tandemly fused to the enhanced green fluorescent protein (EGFP) and the red fluorescent protein (RFP), while the protease was the site-specific protease VP24 from the herpes simplex virus type 1 (HSV-1). The fluorescent proteins in the Rev fusion protein were separated by a cleavage site specific for the VP24 protease. When co-expressed in COS-7 cells proteolysis was observed by fluorescence microscopy as a shift from a predominantly cytoplasmic localization of the fusion protein RevEGFP to a nuclear localization while the RFP part of the fusion protein remained in the cytoplasm. The cleavage of the fusion protein by VP24 was confirmed by Western blot analysis. The activity of VP24, when tagged N-terminally by the Myc-epitope, was found to be comparable to VP24. These results demonstrates that the activity and localization of a recombinantly expressed protease can be assessed by protease-mediated cleavage of fusion proteins containing a specific protease cleavage site.
CLSM, confocal laser scanning microscopy; EGFP, enhanced green fluorescent protein; Green fluorescent protein; HIV, human immunodeficiency virus type 1; HIV-1 Rev; HSV-1 protease; HSV-1, herpes simplex virus type 1; HTLV-1, human T-cell leukaemia virus type 1; NES, nuclear export signal; NLS, nuclear localization signal; NOS, nucleolar localization signal; RFP, red fluorescent protein; Red fluorescent protein
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01-internacional
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MEDLINE
Idioma:
En
Ano de publicação:
2016
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Article