Carbene Footprinting Reveals Binding Interfaces of a Multimeric Membrane-Spanning Protein.
Angew Chem Int Ed Engl
; 56(47): 14873-14877, 2017 11 20.
Article
em En
| MEDLINE
| ID: mdl-28960650
ABSTRACT
Mapping the interaction sites between membrane-spanning proteins is a key challenge in structural biology. In this study a carbene-footprinting approach was developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine-based footprinting probe is effectively sequestered by, and incorporated into, the micelles, thus leading to efficient labelling of the membrane-spanning regions of the protein upon irradiation at 349â
nm. Areas associated with protein-protein interactions between the trimer subunits remained unlabelled, thus revealing their location.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana
/
Metano
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article