Enabling reproducible real-time quantitative PCR research: the RDML package.
Bioinformatics
; 33(24): 4012-4014, 2017 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-28961912
ABSTRACT
MOTIVATION Reproducibility, a cornerstone of research, requires defined data formats, which include the setup and output of experiments. The real-time PCR data markup language (RDML) is a recommended standard of the minimum information for publication of quantitative real-time PCR experiments guidelines. Despite the popularity of the RDML format for analysis of quantitative PCR data, handling of RDML files is not yet widely supported in all PCR curve analysis softwares. RESULTS:
This study describes the open-source RDML package for the statistical computing language R. RDML is compatible with RDML versions ≤ 1.2 and provides functionality to (i) import RDML data; (ii) extract sample information (e.g. targets and concentration); (iii) transform data to various formats of the R environment; (iv) generate human-readable run summaries; and (v) to create RDML files from user data. In addition, RDML offers a graphical user interface to read, edit and create RDML files. AVAILABILITY AND IMPLEMENTATION https//cran.r-project.org/package=RDML. rdmlEdit server http//shtest.evrogen.net/rdmlEdit/. Documentation http//kablag.github.io/RDML/. CONTACT k.blag@yandex.ru. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Reação em Cadeia da Polimerase em Tempo Real
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article