Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes.
Biophys Chem
; 230: 62-67, 2017 11.
Article
em En
| MEDLINE
| ID: mdl-28965786
ABSTRACT
Cy3 and Cy5 dyes linked to the 5' end of a double stranded DNA molecule are known to attach to both strands in a way that is very similar to an additional base pair and has a stabilizing effect on the oligonucleotide. Here we adapt the Peyrard-Bishop mesoscopic model to incorporate cyanine dyes and use the technique of thermal equivalence to obtain the appropriate parameters from existing melting temperatures. We have found that the stacking parameters are in the same range of ordinary AT and CG base pairs, in particular Cy3-A was found to be most rigidly stacked. While the cyanines stabilize the AT hydrogen bonds quite strongly the CG bonds are mostly unaffected.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Carbocianinas
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article