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Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA.
Chen, Jin; Tang, Qingnan; Guo, Shiwen; Lu, Chen; Le, Shimin; Yan, Jie.
Afiliação
  • Chen J; Mechanobiology Institute, National University of Singapore, 117411, Singapore.
  • Tang Q; Department of Physics, National University of Singapore, 117542, Singapore.
  • Guo S; Mechanobiology Institute, National University of Singapore, 117411, Singapore.
  • Lu C; Mechanobiology Institute, National University of Singapore, 117411, Singapore.
  • Le S; Centre for Bioimaging Sciences, National University of Singapore, 117546, Singapore.
  • Yan J; Mechanobiology Institute, National University of Singapore, 117411, Singapore.
Nucleic Acids Res ; 45(17): 10032-10041, 2017 Sep 29.
Article em En | MEDLINE | ID: mdl-28973442
The interaction between the single-stranded DNA and the homologous duplex DNA is essential for DNA homologous repair. Here, we report that parallel triplex structure can form spontaneously between a mechanically extended ssDNA and a homologous dsDNA in protein-free condition. The triplex has a contour length close to that of a B-form DNA duplex and remains stable after force is released. The binding energy between the ssDNA and the homologous dsDNA in the triplex is estimated to be comparable to the basepairing energy in a B-form dsDNA. As ssDNA is in a similar extended conformation within recombinase-coated nucleoprotein filaments, we propose that the parallel triplex may form and serve as an intermediate during recombinase-catalyzed homologous joint formation.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / DNA de Cadeia Simples / Reparo do DNA / DNA de Forma B / Conformação de Ácido Nucleico Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / DNA de Cadeia Simples / Reparo do DNA / DNA de Forma B / Conformação de Ácido Nucleico Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article