Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria.
J Am Chem Soc
; 139(44): 15907-15913, 2017 11 08.
Article
em En
| MEDLINE
| ID: mdl-28976195
ABSTRACT
Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable RuII cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above â¼20 µM, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells. In particular, due to its vastly improved photostability compared to that of conventional SR probes, it can provide images of nuclear DNA at unprecedented resolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
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Microscopia Eletrônica de Transmissão
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Imagem Multimodal
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Metais
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Microscopia de Fluorescência
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Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article