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Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques.
Magnani, Diogo M; Rogers, Thomas F; Beutler, Nathan; Ricciardi, Michael J; Bailey, Varian K; Gonzalez-Nieto, Lucas; Briney, Bryan; Sok, Devin; Le, Khoa; Strubel, Alexander; Gutman, Martin J; Pedreño-Lopez, Núria; Grubaugh, Nathan D; Silveira, Cassia G T; Maxwell, Helen S; Domingues, Aline; Martins, Mauricio A; Lee, David E; Okwuazi, Erica E; Jean, Sherrie; Strobert, Elizabeth A; Chahroudi, Ann; Silvestri, Guido; Vanderford, Thomas H; Kallas, Esper G; Desrosiers, Ronald C; Bonaldo, Myrna C; Whitehead, Stephen S; Burton, Dennis R; Watkins, David I.
Afiliação
  • Magnani DM; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Rogers TF; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Beutler N; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Ricciardi MJ; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Bailey VK; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Gonzalez-Nieto L; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Briney B; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Sok D; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Le K; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Strubel A; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Gutman MJ; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Pedreño-Lopez N; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Grubaugh ND; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Silveira CGT; Division of Clinical Immunology and Allergy, School of Medicine, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Maxwell HS; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Domingues A; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Martins MA; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Lee DE; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Okwuazi EE; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Jean S; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Strobert EA; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Chahroudi A; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Silvestri G; Department of Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Vanderford TH; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Kallas EG; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
  • Desrosiers RC; Division of Clinical Immunology and Allergy, School of Medicine, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Bonaldo MC; Department of Pathology, University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136, USA.
  • Whitehead SS; Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, Brazil.
  • Burton DR; Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Watkins DI; Department of Immunology and Microbiology and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA. burton@scripps.edu dwatkins@med.miami.edu.
Sci Transl Med ; 9(410)2017 Oct 04.
Article em En | MEDLINE | ID: mdl-28978754
ABSTRACT
Therapies to prevent maternal Zika virus (ZIKV) infection and its subsequent fetal developmental complications are urgently required. We isolated three potent ZIKV-neutralizing monoclonal antibodies (nmAbs) from the plasmablasts of a ZIKV-infected patient-SMZAb1, SMZAb2, and SMZAb5-directed against two different domains of the virus. We engineered these nmAbs with Fc LALA mutations that abrogate Fcγ receptor binding, thus eliminating potential therapy-mediated antibody-dependent enhancement. We administered a cocktail of these three nmAbs to nonhuman primates 1 day before challenge with ZIKV and demonstrated that the nmAbs completely prevented viremia in serum after challenge. Given that numerous antibodies have exceptional safety profiles in humans, the cocktail described here could be rapidly developed to protect uninfected pregnant women and their fetuses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Neutralizantes / Infecção por Zika virus / Anticorpos Monoclonais Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Neutralizantes / Infecção por Zika virus / Anticorpos Monoclonais Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article