Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy.
Photodiagnosis Photodyn Ther
; 20: 201-206, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-29042307
ABSTRACT
Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365-490nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Luz
/
Microscopia de Fluorescência
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article