Production of steviol from steviol glucosides using ß-glucosidase from a commercial pectinase, Sumizyme PX.
Biotechnol Lett
; 40(1): 197-204, 2018 Jan.
Article
em En
| MEDLINE
| ID: mdl-29064007
ABSTRACT
OBJECTIVE:
To purify and characterize a specific enzyme from a commercial pectinase for the production of steviol from stevioside (Ste) without adding organic solvent and to improve steviol production.RESULTS:
Commercial Sumizyme PX converted Ste to steviol with a yield of 98%. ß-Glucosidase from Sumizyme PX (ßglyPX) was purified in three steps with 12.5-fold purification and 51% yield. The specific activity of the purified ßglyPX was 141 U/mg. The molecular weight of ßglyPX was ~ 116 kDa on SDS-PAGE. Its optimum activity was at pH 3.5 and 65 °C. It was stable for 12 h up to 55 °C and for 24 h at pH 2-9.5. K m values of ßglyPX for pNPGal, oNPGlc, lactose, and Ste were 2.4, 0.7, 18, and 7.8 mM, respectively. The optimum conditions for steviol production were 55 °C, 900 U/ml, 80 mg Ste/ml, 12 h.CONCLUSION:
ßglyPX contains great potential for industrial steviol production from Ste.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poligalacturonase
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Beta-Glucosidase
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Diterpenos do Tipo Caurano
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Glucosídeos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article