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Isolation, cultivation, and characterization of human mesenchymal stem cells.
Mushahary, Dolly; Spittler, Andreas; Kasper, Cornelia; Weber, Viktoria; Charwat, Verena.
Afiliação
  • Mushahary D; Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
  • Spittler A; Core Facility Flow Cytometry & Surgical Research Laboratories, Medical University of Vienna, 1090 Vienna, Austria.
  • Kasper C; Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
  • Weber V; Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Danube University Krems, 3500 Krems, Austria.
  • Charwat V; Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
Cytometry A ; 93(1): 19-31, 2018 01.
Article em En | MEDLINE | ID: mdl-29072818
ABSTRACT
Mesenchymal stem cells (MSC) exhibit a high self-renewal capacity, multilineage differentiation potential and immunomodulatory properties. This set of exceptional features makes them an attractive tool for research and clinical application. However, MSC are far from being a uniform cell type, which makes standardization difficult. The exact properties of human MSC (hMSC) can vary greatly depending on multiple parameters including tissue source, isolation method and medium composition. In this review we address the most important influence factors. We highlight variations in the differentiation potential of MSC from different tissue sources. Furthermore, we compare enzymatic isolation strategies with explants cultures focusing on adipose tissue and umbilical cords as two relevant examples. Additionally, we address effects of medium composition and serum supplementation on MSC expansion and differentiation. The lack of standardized methods for hMSC isolation and cultivation mandates careful evaluation of different protocols regarding efficiency and cell quality. MSC characterization based on a set of minimal criteria defined by the International Society for Cellular Therapy is a widely accepted practice, and additional testing for MSC functionality can provide valuable supplementary information. The MSC secretome has been identified as an important signaling mechanism to affect other cells. In this context, extracellular vesicles (EVs) are attracting increasing interest. The thorough characterization of MSC-derived EVs and their interaction with target cells is a crucial step toward a more complete understanding of MSC-derived EV functionality. Here, we focus on flow cytometric approaches to characterize free as well as cell bound EVs and address potential differences in the bioactivity of EVs derived from stem cells from different sources. © 2017 International Society for Advancement of Cytometry.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Mesenquimais Tipo de estudo: Guideline / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Mesenquimais Tipo de estudo: Guideline / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article