Quantification of sulphur amino acids by ultra-high performance liquid chromatography in aquatic invertebrates.
Anal Biochem
; 539: 158-161, 2017 12 15.
Article
em En
| MEDLINE
| ID: mdl-29106907
ABSTRACT
We examined the performance of an ultra-high performance liquid chromatography method to quantify protein-bound sulphur amino acids in zooplankton. Both cysteic acid and methionine sulfone were linear from 5 to 250 pmol (r2 = 0.99), with a method detection limit of 13 pmol and 9 pmol, respectively. Although there was no matrix effect on linearity, adjacent peaks and co-eluting noise from the invertebrate proteins increased the detection limits when compared to common standards. Overall, performance characteristics were reproducible and accurate, and provide a means for quantifying sulphur amino acids in aquatic invertebrates, an understudied group.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zooplâncton
/
Cromatografia Líquida de Alta Pressão
/
Aminoácidos Sulfúricos
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article