Spata-13,17-diene Synthase-An Enzyme with Sesqui-, Di-, and Sesterterpene Synthase Activity from Streptomyces xinghaiensis.
Angew Chem Int Ed Engl
; 56(51): 16385-16389, 2017 12 18.
Article
em En
| MEDLINE
| ID: mdl-29125678
ABSTRACT
A terpene synthase from the marine bacterium Streptomyces xinghaiensis has been characterised, including a full structure elucidation of its products from various substrates and an in-depth investigation of the enzyme mechanism by isotope labelling experiments, metal cofactor variations, and mutation experiments. The results revealed an interesting dependency of Mn2+ catalysis on the presence of Asp-217, a residue that is occupied by a highly conserved Glu in most other bacterial terpene synthases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Alquil e Aril Transferases
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article