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Non-monotonic Changes in Progenitor Cell Behavior and Gene Expression during Aging of the Adult V-SVZ Neural Stem Cell Niche.
Apostolopoulou, Maria; Kiehl, Thomas R; Winter, Mark; Cardenas De La Hoz, Edgar; Boles, Nathan C; Bjornsson, Christopher S; Zuloaga, Kristen L; Goderie, Susan K; Wang, Yue; Cohen, Andrew R; Temple, Sally.
Afiliação
  • Apostolopoulou M; Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
  • Kiehl TR; Neural Stem Cell Institute, Rensselaer, NY 12144, USA. Electronic address: tomkiehl@neuralsci.org.
  • Winter M; Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Cardenas De La Hoz E; Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Boles NC; Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
  • Bjornsson CS; Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
  • Zuloaga KL; Neural Stem Cell Institute, Rensselaer, NY 12144, USA; Department of Neuroscience and Experimental Therapeutics, Albany Medical College, Albany, NY 12208, USA.
  • Goderie SK; Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
  • Wang Y; Neural Stem Cell Institute, Rensselaer, NY 12144, USA.
  • Cohen AR; Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Temple S; Neural Stem Cell Institute, Rensselaer, NY 12144, USA. Electronic address: sallytemple@neuralsci.org.
Stem Cell Reports ; 9(6): 1931-1947, 2017 12 12.
Article em En | MEDLINE | ID: mdl-29129683
Neural stem cell activity in the ventricular-subventricular zone (V-SVZ) decreases with aging, thought to occur by a unidirectional decline. However, by analyzing the V-SVZ transcriptome of male mice at 2, 6, 18, and 22 months, we found that most of the genes that change significantly over time show a reversal of trend, with a maximum or minimum expression at 18 months. In vivo, MASH1+ progenitor cells decreased in number and proliferation between 2 and 18 months but increased between 18 and 22 months. Time-lapse lineage analysis of 944 V-SVZ cells showed that age-related declines in neurogenesis were recapitulated in vitro in clones. However, activated type B/type C cell clones divide slower at 2 to 18 months, then unexpectedly faster at 22 months, with impaired transition to type A neuroblasts. Our findings indicate that aging of the V-SVZ involves significant non-monotonic changes that are programmed within progenitor cells and are observable independent of the aging niche.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Envelhecimento / Neurogênese / Células-Tronco Neurais / Transcriptoma Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Envelhecimento / Neurogênese / Células-Tronco Neurais / Transcriptoma Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article