UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods.
Methods Mol Biol
; 1980: 223-232, 2020.
Article
em En
| MEDLINE
| ID: mdl-29159730
ABSTRACT
Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteróis
/
Cromatografia Líquida de Alta Pressão
/
Ácidos Graxos não Esterificados
/
Lipídeos
/
Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article