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Structure of PINK1 in complex with its substrate ubiquitin.
Schubert, Alexander F; Gladkova, Christina; Pardon, Els; Wagstaff, Jane L; Freund, Stefan M V; Steyaert, Jan; Maslen, Sarah L; Komander, David.
Afiliação
  • Schubert AF; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Gladkova C; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Pardon E; VIB-VUB Center for Structural Biology, VIB, 1050 Brussels, Belgium.
  • Wagstaff JL; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
  • Freund SMV; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Steyaert J; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Maslen SL; VIB-VUB Center for Structural Biology, VIB, 1050 Brussels, Belgium.
  • Komander D; Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
Nature ; 552(7683): 51-56, 2017 12 07.
Article em En | MEDLINE | ID: mdl-29160309
Autosomal-recessive juvenile Parkinsonism (AR-JP) is caused by mutations in a number of PARK genes, in particular the genes encoding the E3 ubiquitin ligase Parkin (PARK2, also known as PRKN) and its upstream protein kinase PINK1 (also known as PARK6). PINK1 phosphorylates both ubiquitin and the ubiquitin-like domain of Parkin on structurally protected Ser65 residues, triggering mitophagy. Here we report a crystal structure of a nanobody-stabilized complex containing Pediculus humanus corporis (Ph)PINK1 bound to ubiquitin in the 'C-terminally retracted' (Ub-CR) conformation. The structure reveals many peculiarities of PINK1, including the architecture of the C-terminal region, and reveals how the N lobe of PINK1 binds ubiquitin via a unique insertion. The flexible Ser65 loop in the Ub-CR conformation contacts the activation segment, facilitating placement of Ser65 in a phosphate-accepting position. The structure also explains how autophosphorylation in the N lobe stabilizes structurally and functionally important insertions, and reveals the molecular basis of AR-JP-causing mutations, some of which disrupt ubiquitin binding.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pediculus / Proteínas Quinases / Ubiquitina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pediculus / Proteínas Quinases / Ubiquitina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article