miR-590-3p promotes colon cancer cell proliferation via Wnt/ß-catenin signaling pathway by inhibiting WIF1 and DKK1.
Eur Rev Med Pharmacol Sci
; 21(21): 4844-4852, 2017 Nov.
Article
em En
| MEDLINE
| ID: mdl-29164578
ABSTRACT
OBJECTIVE:
Colon cancer is one of the most common and deadly types of gastrointestinal tumor. Despite progressive treatments, the patient prognosis has not been improved effectively. MATERIALS ANDMETHODS:
Expression of miRNA and mRNA were tested by Realtime PCR. Cell cycle was detected by flow cytometry. Cell viability was evaluated by MTT assay. Cell spheroid formation was determined by colony assay. Wnt signaling pathway activity was evaluated by TOP/FOP ratio. Protein expression was tested using Western blot. ß-catenin binding ability was detected by ChIP assay. miRNA target gene was confirmed by luciferase assay.RESULTS:
miR-590-3p was found to be overexpressed in both glioma tissues and cell lines. miR-590-3p is upregulated in colon cancer cells and tissues compared to non-tumorigenic colon cells and normal colon tissues. miR-590-3p positively regulated cell proliferation, spheroid formation, and cell cycle in LS174T cells. Conversely, inhibition of miR-590-3p reduced these effects. We confirmed that WIF1 and DKK1 are targets of miR-590-3p. Overexpression of miR-590-3p promoted TOP flash luciferase activity, enhanced nuclear ß-catenin levels and increased target genes expression of Wnt signaling pathway. The results indicated that miR-590-3p activates the Wnt/ß-catenin signaling pathway.CONCLUSIONS:
We demonstrate that miR-590-3p regulates colon cancer progression via WIF1 and DKK1, which suggests that miR-590-3p may be a promising candidate for therapeutic applications in colon cancer treatment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Neoplasias do Colo
/
Peptídeos e Proteínas de Sinalização Intercelular
/
MicroRNAs
/
Proteínas Adaptadoras de Transdução de Sinal
/
Via de Sinalização Wnt
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article