Quantitative Visualization of Nanoscale Ion Transport.
Anal Chem
; 89(24): 13603-13609, 2017 12 19.
Article
em En
| MEDLINE
| ID: mdl-29164870
ABSTRACT
Understanding ion transport properties at various interfaces, especially at small length scales, is critical in advancing our knowledge of membrane materials and cell biology. Recently, we described potentiometric-scanning ion conductance microscopy (P-SICM) for ion-conductance measurement in polymer membranes and epithelial cell monolayers at discrete points in a sample. Here, we combine hopping mode techniques with P-SICM to allow simultaneous nanometer-scale conductance and topography mapping. First validated with standard synthetic membranes and then demonstrated in living epithelial cell monolayers under physiological conditions, this new method allows direct visualization of heterogeneous ion transport of biological samples for the first time. These advances provide a noncontact local probe, require no labeling, and present a new tool for quantifying intrinsic transport properties of a variety of biological samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Transporte de Íons
/
Células Madin Darby de Rim Canino
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article