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Injury Activates Transient Olfactory Stem Cell States with Diverse Lineage Capacities.
Gadye, Levi; Das, Diya; Sanchez, Michael A; Street, Kelly; Baudhuin, Ariane; Wagner, Allon; Cole, Michael B; Choi, Yoon Gi; Yosef, Nir; Purdom, Elizabeth; Dudoit, Sandrine; Risso, Davide; Ngai, John; Fletcher, Russell B.
Afiliação
  • Gadye L; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Das D; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Berkeley Institute for Data Science, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Sanchez MA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Street K; Division of Biostatistics, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Baudhuin A; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Wagner A; Department of Electrical Engineering and Computer Science, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Cole MB; Department of Physics, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Choi YG; QB3 Functional Genomics Laboratory, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Yosef N; Department of Electrical Engineering and Computer Science, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Purdom E; Department of Statistics, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Dudoit S; Berkeley Institute for Data Science, University of California, Berkeley, Berkeley, CA 94720, USA; Division of Biostatistics, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Statistics, University of California, Berkeley, Berkeley, CA 94720, USA; Center for Computational Bi
  • Risso D; Division of Biostatistics, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Healthcare Policy and Research, Weill Cornell Medical College, New York, NY 10065, USA.
  • Ngai J; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; QB3 Functional Genomics Laboratory, University of California, Berkeley, Berkeley, CA 94720, USA. Ele
  • Fletcher RB; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell Stem Cell ; 21(6): 775-790.e9, 2017 Dec 07.
Article em En | MEDLINE | ID: mdl-29174333
ABSTRACT
Tissue homeostasis and regeneration are mediated by programs of adult stem cell renewal and differentiation. However, the mechanisms that regulate stem cell fates under such widely varying conditions are not fully understood. Using single-cell techniques, we assessed the transcriptional changes associated with stem cell self-renewal and differentiation and followed the maturation of stem cell-derived clones using sparse lineage tracing in the regenerating mouse olfactory epithelium. Following injury, quiescent olfactory stem cells rapidly shift to activated, transient states unique to regeneration and tailored to meet the demands of injury-induced repair, including barrier formation and proliferation. Multiple cell fates, including renewed stem cells and committed differentiating progenitors, are specified during this early window of activation. We further show that Sox2 is essential for cells to transition from the activated to neuronal progenitor states. Our study highlights strategies for stem cell-mediated regeneration that may be conserved in other adult stem cell niches.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Mucosa Olfatória / Linhagem da Célula Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Mucosa Olfatória / Linhagem da Célula Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article