3D microniches reveal the importance of cell size and shape.
Nat Commun
; 8(1): 1962, 2017 12 06.
Article
em En
| MEDLINE
| ID: mdl-29213086
ABSTRACT
Geometrical cues have been shown to alter gene expression and differentiation on 2D substrates. However, little is known about how geometrical cues affect cell function in 3D. One major reason for this lack of understanding is rooted in the difficulties of controlling cell geometry in a complex 3D setting and for long periods of culture. Here, we present a robust method to control cell volume and shape of individual human mesenchymal stem cells (hMSCs) inside 3D microniches with a range of different geometries (e.g., cylinder, triangular prism, cubic, and cuboid). We find that the actin filaments, focal adhesions, nuclear shape, YAP/TAZ localization, cell contractility, nuclear accumulation of histone deacetylase 3, and lineage selection are all sensitive to cell volume. Our 3D microniches enable fundamental studies on the impact of biophysical cues on cell fate, and have potential applications in investigating how multicellular architectures organize within geometrically well-defined 3D spaces.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Técnicas Citológicas
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Técnicas de Cultura de Células
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Forma Celular
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Tamanho Celular
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Células-Tronco Mesenquimais
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Microambiente Celular
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article