[Screening of Differential Expression Proteins in Rat Brain Tissues after DAI by iTRAQ-LC-MS/MS].
Fa Yi Xue Za Zhi
; 33(4): 348-352, 2017 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-29219262
ABSTRACT
OBJECTIVES:
To screen for the differential expression proteins in brain tissues of SD rat after diffuse axonal injury ï¼DAIï¼ by isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer/mass spectrometer ï¼iTRAQ-LC-MS/MSï¼, and to explore potential biomarkers available for the diagnosis of DAI.METHODS:
Animal models of DAI were established with the Marmarou method as reference, and the subjects were divided into blank control group ï¼n=4ï¼, sham strike group ï¼n=4ï¼ and fatal strike group ï¼n=4ï¼, respectively. The proteins in rat brain tissues were detected by iTRAQ-LC-MS/MS, and bioinformatics analysis and verification were performed on the results and screened for the differential expression proteins.RESULTS:
A total of 2 016 proteins were identified and quantified. The bioinformatics analysis revealed that the proteins had wide distribution and function, and participated in different biological processes. There were 16 proteins showed differential expression in fatal strike group, including one up-regulated expression protein and 15 down-regulated expression proteins. The results of iTRAQ-LC-MS/MS were confirmed by Western blotting method.CONCLUSIONS:
Multiple differential expression proteins in rat brain tissues after DAI can be screened by iTRAQ-LC-MS/MS. This not only indicates a research direction for exploring the pathogenesis of DAI, but also provides potential biomarkers available for the diagnosis of DAI.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesão Axonal Difusa
/
Proteômica
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article