Recreating Intestinal Peristalsis in the Petri Dish.
Methods Mol Biol
; 1727: 423-432, 2018.
Article
em En
| MEDLINE
| ID: mdl-29222802
ABSTRACT
Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic reflex. After a few days in culture, these cells reorganize to form a small group of cells that permit the generation of pacemaker activity, spontaneous contractions, and the development of inhibitory and excitatory junction potentials in the petri dish, all elements involved in the peristaltic reflex. Therefore, these co-cultures are suitable to study the cellular and molecular aspects related to the development, maintenance, and modulation of motor intestinal functions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cocultura
/
Miócitos de Músculo Liso
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Intestino Delgado
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Neurônios Motores
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article