Time-dependent Expression of MT1A mRNA and MT2A mRNA in the Contused Skeletal Muscle of Rats.
Fa Yi Xue Za Zhi
; 33(1): 6-10, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-29231000
ABSTRACT
OBJECTIVES:
To investigate the time-dependent expression of metallothionein ï¼MTï¼ 1A mRNA and MT2A mRNA in contused skeletal muscle of rats.METHODS:
A total of 54 Sprague-Dawley rats were used in this study. The rats were divided into two parts control group ï¼n=6ï¼ and contusion groups ï¼0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, n=6ï¼. Total RNA was extracted from skeletal muscle. The expression levels of MT1A mRNA and MT2A mRNA were detected by SYBR Green I real-time PCR.RESULTS:
The expression trends of the two potential marker genes were related to wound age. In addition to 0.5 h, there were significant contrasts between the control group and contused group ï¼P<0.05ï¼, about the expression levels of MT1A mRNA and MT2A mRNA in different phases. As the extension of wound age, the relative expression of MT1A mRNA and MT2A mRNA at 1 h, 6 h, 12 h and 18 h after contusion demonstrated upgrade tendency until its expression levels in 18 h peak with 239.41±15.20 and 717.42±50.76, respectively. When time extends to 24 h after injury, the expression of above two marks decreased, respectively. The MT1A mRNA and MT2A mRNA expression levels increased at 30 h and then decreased.CONCLUSIONS:
Determination of MT1A mRNA and MT2A mRNA levels by real-time PCR may be useful for the estimation of wound age.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Músculo Esquelético
/
Contusões
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article