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Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria.
Bernheim, Aude; Calvo-Villamañán, Alicia; Basier, Clovis; Cui, Lun; Rocha, Eduardo P C; Touchon, Marie; Bikard, David.
Afiliação
  • Bernheim A; Microbial Evolutionary Genomics, Institut Pasteur, 25-28 rue Dr. Roux, 75015, Paris, France.
  • Calvo-Villamañán A; CNRS, UMR3525, 25-28 rue Dr. Roux, 75015, Paris, France.
  • Basier C; Synthetic Biology Group, Institut Pasteur, 25-28 rue Dr. Roux, 75015, Paris, France.
  • Cui L; AgroParisTech, 75005, Paris, France.
  • Rocha EPC; Synthetic Biology Group, Institut Pasteur, 25-28 rue Dr. Roux, 75015, Paris, France.
  • Touchon M; Synthetic Biology Group, Institut Pasteur, 25-28 rue Dr. Roux, 75015, Paris, France.
  • Bikard D; Synthetic Biology Group, Institut Pasteur, 25-28 rue Dr. Roux, 75015, Paris, France.
Nat Commun ; 8(1): 2094, 2017 12 12.
Article em En | MEDLINE | ID: mdl-29234047
Type II CRISPR-Cas systems introduce double-strand breaks into DNA of invading genetic material and use DNA fragments to acquire novel spacers during adaptation. These breaks can be the substrate of several DNA repair pathways, paving the way for interactions. We report that non-homologous end-joining (NHEJ) and type II-A CRISPR-Cas systems only co-occur once among 5563 fully sequenced prokaryotic genomes. We investigated experimentally the possible molecular interactions using the NHEJ pathway from Bacillus subtilis and the type II-A CRISPR-Cas systems from Streptococcus thermophilus and Streptococcus pyogenes. Our results suggest that the NHEJ system has no effect on CRISPR immunity. On the other hand, we provide evidence for the inhibition of NHEJ repair by the Csn2 protein. Our findings give insights on the complex interactions between CRISPR-Cas systems and repair mechanisms in bacteria, contributing to explain the scattered distribution of CRISPR-Cas systems in bacterial genome.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Genoma Bacteriano / Reparo do DNA por Junção de Extremidades / Proteínas Associadas a CRISPR / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Genoma Bacteriano / Reparo do DNA por Junção de Extremidades / Proteínas Associadas a CRISPR / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2017 Tipo de documento: Article