Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon.
Bioorg Med Chem Lett
; 28(2): 77-80, 2018 01 15.
Article
em En
| MEDLINE
| ID: mdl-29248297
ABSTRACT
We have developed RNA-based quencher-free molecular aptamer beacons (RNA-based QF-MABs) for the detection of ATP, taking advantage of the conformational changes associated with ATP binding to the ATP-binding RNA aptamer. The RNA aptamer, with its well-defined structure, was readily converted to the fluorescence sensors by incorporating a fluorophore into the loop region of the hairpin structure. These RNA-based QF-MABs exhibited fluorescence signals in the presence of ATP relative to their low background signals in the absence of ATP. The fluorescence emission intensity increased upon formation of a RNA-based QF-MAB·ATP complex.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
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Trifosfato de Adenosina
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Aptâmeros de Nucleotídeos
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article