Flavobacterium phocarum sp. nov., isolated from soils of a seal habitat in Antarctica.
Int J Syst Evol Microbiol
; 68(2): 536-541, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29251588
ABSTRACT
A Gram-stain-negative, yellow-pigmented, non-flagellated, gliding, rod-shaped, oxidase-negative and catalase-positive bacterium, designated SE14T, was isolated from soil on King George Island, South Shetland Islands, Antarctica. Strain SE14T grew at 4-25 °C (optimum, 20 °C), at pH 6.0-9.0 (optimum, pH 7.0-7.5) and with 0-3.0â% NaCl (optimum, 1.0-1.5â%), and could not produce flexirubin-type pigments. 16S rRNA gene sequence analysis showed the the isolate belonged to the genus Flavobacterium. Strain SE14T had the highest 16S rRNA gene sequence similarity to Flavobacterium antarcticum, F. tegetincola and F. degerlachei with 95.8, 95.5 and 95.2â%, respectively. The strain SE14T consisted of a clade with Flavobacteriumnoncentrifugens (16S rRNA gene sequence similarity 94.9â%) and F. qiangtangense (16S rRNA gene sequence similarity 94.2â%) and simultaneously formed a distinct phyletic lineage in the neighbour-joining phylogenetic tree. Polar lipids of the strain included phosphatidylethanolamine and four unidentified aminolipids. Strain SE14T contained anteiso-C15ââ0, iso-C15ââ0 and a mixture of iso-C15ââ0 2-OH and/or C16ââ1ω7c as the main fatty acids, and the only respiratory quinone was menaquinone-6. The genomic DNA G+C content was 42.3 mol%. The polyphasic taxonomic study revealed that strain SE14T belongs to a novel species within the genus Flavobacterium , and the name Flavobacterium phocarum sp. nov. is proposed. The type strain is SE14T (=CCTCC AB 2017225T=KCTC 52612T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Microbiologia do Solo
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Flavobacterium
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Focas Verdadeiras
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article