Role of protein delta homolog 1 in the proliferation and differentiation of ameloblasts.

Qi, Shengcai; Zhu, Xueqin; Wang, Xiaoning; Chen, Fubo; Yan, Yanhong; Shang, Guangwei; Chen, Wantao

Qi, Shengcai; Zhu, Xueqin; Wang, Xiaoning; Chen, Fubo; Yan, Yanhong; Shang, Guangwei; Chen, Wantao.

Afiliação

Qi S; Department of Oral and MaxillofacialHead and Neck Oncology, and Faculty of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
Zhu X; Department of Oral and MaxillofacialHead and Neck Oncology, and Faculty of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
Wang X; Department of Oral and MaxillofacialHead and Neck Oncology, and Faculty of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
Chen F; Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China.
Yan Y; Department of Pediatric Dentistry, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
Shang G; Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China.
Chen W; Department of Oral and MaxillofacialHead and Neck Oncology, and Faculty of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.

Mol Med Rep ; 17(3): 3537-3544, 2018 03.

Article em En | MEDLINE | ID: mdl-29257328

ABSTRACT

ABSTRACT

Protein delta homolog 1 (DLK1) regulates the odontoblastic differentiation of human dental pulp stem cells. It was hypothesized that DLK1 may exert regulatory effects on epithelialmesenchymal interactions in tooth development. The present study investigated the expression of DLK1 during the development of mouse enamel and its role in the proliferation and differentiation of ameloblastlineage cells (ALCs). DLK1 expression was upregulated in ameloblasts in the first mandibular molar during the entire process of enamel development. The mRNA and protein levels of DLK1 were significantly upregulated following ameloblastic induction in ALCs. In addition, overexpression of DLK1 promoted the proliferation of ALCs, inhibited ameloblastic differentiation, upregulated the expression of amelogenin and enamelin, and downregulated the expression of odontogenic ameloblastassociated protein and kallikrein 4. The results of the present study suggested that DLK1 may be a potent regulator of ameloblast proliferation and differentiation, and may regulate enamel formation during tooth development.

Assuntos

Diferenciação Celular; Proliferação de Células; Peptídeos e Proteínas de Sinalização Intercelular/metabolismo; Ameloblastos/citologia; Ameloblastos/metabolismo; Amelogenina/genética; Amelogenina/metabolismo; Animais; Proteínas de Ligação ao Cálcio; Linhagem Celular; Esmalte Dentário/crescimento & desenvolvimento; Esmalte Dentário/metabolismo; Esmalte Dentário/patologia; Proteínas do Esmalte Dentário/genética; Proteínas do Esmalte Dentário/metabolismo; Feminino; Peptídeos e Proteínas de Sinalização Intercelular/genética; Calicreínas/genética; Calicreínas/metabolismo; Masculino; Camundongos; Camundongos Endogâmicos C57BL; RNA Mensageiro/metabolismo

Palavras-chave

protein delta homolog 1; enamel; ameloblast-lineage cell; proliferation; ameloblastic differentiation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Peptídeos e Proteínas de Sinalização Intercelular / Proliferação de Células Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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