[Analysis of Irregular Antibody Production in Severe ß-Thalassemia Children with long-term Blood Transtusion and Its Correlation with RH Gene and Anemia Gene Mutation Site].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 25(6): 1756-1760, 2017 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-29262911
ABSTRACT
OBJECTIVE:
To analyze the prevalence of irregular antibodies in children with severe ß-thalassemia after long-term blood transfusion and its correlation with RH and anemia gene mutations site.METHODS:
One hundred twenty children with severe ß-thalassemia and long-term blood transfusion were selected in our hospital, the genomic DNA was extracted and the genotype of RH factor were determined by PCR-SSP. The irregular antibodies and their types were screened and identified by the serological method, the gene types of the severe ß-thalassemia were analyzed by reverse dot blot hybridization on DNA chip and PCR amplification.RESULTS:
The major of RH genotypes in 120 children were Ce/Ce (59.17%) and CE/ce (25%), among them 10 children possessed the positive irregular antibodies (8.33%), out of these 10 children, the genotypes of RH factor were Ce/Ce in 6 cases, cE/cE, CE/ce, cE/ce and Ce/ce in one case; among these 10 children with positive irregular antibodies, the anemia gene mutations were IVs-11654M in 2 cases, cD4142M in 6 cases, 28M in 1 case, and CD71-72M in 1 case.CONCLUSION:
Irregular antibodies produced by regular blood transfusion in children with severe ß-thalassemia may be related with RH factor and anemia gene mutation sites.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema do Grupo Sanguíneo Rh-Hr
/
Talassemia beta
/
Formação de Anticorpos
/
Mutação
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Child
/
Humans
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article