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High-performance liquid chromatography study of gatifloxacin and sparfloxacin using erythrosine as post-column resonance Rayleigh scattering reagent and mechanism study.
Pan, Ziyu; Peng, Jingdong; Zang, Xu; Peng, Huanjun; Xiao, Huan; Bu, Lingli; Chen, Fang; He, Yan; Chen, Yu; Wang, Xiang; Li, Shiyu; Chen, Yi.
Afiliação
  • Pan Z; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Peng J; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Zang X; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Peng H; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Xiao H; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Bu L; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Chen F; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • He Y; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Chen Y; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Wang X; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Li S; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
  • Chen Y; School of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China.
Luminescence ; 33(2): 417-424, 2018 Mar.
Article em En | MEDLINE | ID: mdl-29271102
ABSTRACT
Herein, a highly selective high-performance liquid chromatography (HPLC) coupled with resonance Rayleigh scattering (RRS) method was developed to detect gatifloxacin (GFLX) and sparfloxacin (SPLX). GFLX and SPLX were first separated by HPLC, then, in pH 4.4 Britton-Robinson (BR) buffer medium, protonic quaternary ammonia cation of GFLX and SPLX reacted with erythrosine (ERY) to form 11 ion-association complexes, which resulted in a significant enhancement of RRS signal. The experimental conditions of HPLC and post-column RRS have been investigated, including detection wavelength, flow rate, pH, reacting tube length and reaction temperature. Reaction mechanism were studied in detail by calculating the distribution fraction. The maximum RRS signals for GFLX and SPLX were recorded at λex  = λem  = 330 nm. The detection limits were 3.8 ng ml-1 for GFLX and 17.5 ng ml-1 for SPLX at a signal-to-noise ratio of 3. The developed method was successfully applied to the determination of GFLX and SPLX in water samples. Recoveries from spiked water samples were 97.56-98.85%.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Fluoroquinolonas / Eritrosina / Antibacterianos Tipo de estudo: Evaluation_studies Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Líquida de Alta Pressão / Fluoroquinolonas / Eritrosina / Antibacterianos Tipo de estudo: Evaluation_studies Idioma: En Ano de publicação: 2018 Tipo de documento: Article