In vivo evidence of inflammasome activation during spontaneous labor at term.
J Matern Fetal Neonatal Med
; 32(12): 1978-1991, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-29295667
ABSTRACT
OBJECTIVE:
Upon inflammasome activation, the adaptor protein of the inflammasome ASC (apoptosis-associated speck-like protein containing a CARD) forms intracellular specks, which can be released into the extracellular space. The objectives of this study were to investigate whether (1) extracellular ASC is present in the amniotic fluid of women who delivered at term; (2) amniotic fluid ASC concentrations are greater in women who underwent spontaneous labor at term than in those who delivered at term in the absence of labor; and (3) amniotic epithelial and mesenchymal cells can form intracellular ASC specks in vitro.METHODS:
This retrospective cross-sectional study included amniotic fluid samples from 41 women who delivered at term in the absence of labor (n = 24) or underwent spontaneous labor at term (n = 17). Amniotic epithelial and mesenchymal cells were also isolated from the chorioamniotic membranes obtained from a separate group of women who delivered at term (n = 3), in which ASC speck formation was assessed by confocal microscopy. Monocytes from healthy individuals were used as positive controls for ASC speck formation (n = 3).RESULTS:
(1) The adaptor protein of the inflammasome ASC is detectable in the amniotic fluid of women who delivered at term; (2) amniotic fluid ASC concentration was higher in women who underwent spontaneous labor at term than in those who delivered at term without labor; and (3) amniotic epithelial and mesenchymal cells are capable of forming ASC specks and/or filaments in vitro.CONCLUSION:
Amniotic fluid ASC concentrations are increased in women who undergo spontaneous labor at term. Amniotic epithelial and mesenchymal cells are capable of forming ASC specks, suggesting that these cells are a source of extracellular ASC in the amniotic fluid. These findings provide in vivo evidence that there is inflammasome activation in the amniotic cavity during the physiological process of labor at term.Palavras-chave
ASC speck; Amnion; PYD and CARD Domain-Containing Protein (Pycard); amniotic fluid; apoptosis-associated speck-like protein containing a CARD (ASC); caspase-1; chorioamniotic membranes; chorion; cytokine; interleukin-18; interleukin-1beta; intra-amniotic inflammation; parturition; pattern recognition receptor (PRR); pregnancy; sterile inflammation
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trabalho de Parto
/
Nascimento a Termo
/
Proteínas Adaptadoras de Sinalização CARD
/
Inflamassomos
/
Líquido Amniótico
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article