Selective Radical Trifluoromethylation of Native Residues in Proteins.
J Am Chem Soc
; 140(5): 1568-1571, 2018 02 07.
Article
em En
| MEDLINE
| ID: mdl-29301396
ABSTRACT
The incorporation of fluorine can not only significantly facilitate the study of proteins but also potentially modulate their function. Though some biosynthetic methods allow global residue-replacement, post-translational fluorine incorporation would constitute a fast and efficient alternative. Here, we reveal a mild method for direct protein radical trifluoromethylation at native residues as a strategy for symmetric-multifluorine incorporation on mg scales with high recoveries. High selectivity toward tryptophan residues enhanced the utility of this direct trifluoromethylation technique allowing ready study of fluorinated protein constructs using 19F-NMR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triptofano
/
Hemeproteínas
/
Hidrocarbonetos Fluorados
/
Mioglobina
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article