The matrix synthesis and anti-inflammatory effect of autologous leukocyte-poor platelet rich plasma in human cartilage explants.
Histol Histopathol
; 33(6): 609-618, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29313321
ABSTRACT
OBJECTIVE:
To determine the effects of autologous leukocyte-poor platelet-rich plasma (LP-PRP) on the expression of markers involved in cartilage-extracellular matrix production and inflammation in cartilage explants bearing osteoarthritis. MATERIALS ANDMETHODS:
Cartilage explants and LP-PRP were obtained from 10 patients who underwent total knee arthroplasty. The explants were cultured in spinner flasks for 28 days in the presence of interleukin (IL)-1ß and/or LP-PRP. The gene expression of catabolic (MMP13, ADAMTS5, and IL1ß) and anabolic factors (COL2A1, ACAN, and SOX9) was quantified. A histological assessment was performed according to a modified Mankin score, and quantification of type II and I collagen deposition.RESULTS:
The gene expression of catabolic factors and the Mankin score were lower in LP-PRP- and LP-PRP/IL-1ß- than in IL-1ß-treated explants, suggesting less matrix degradation in explants cultured in the presence of LP-PRP. Higher expression of genes involved in cartilage matrix restoration was observed in LP-PRP and LP-PRP/IL-1ß- when compared to IL-1ß-treated explants. The explants treated with LP-PRP and LP-PRP/IL-1ß exhibited a higher deposition of type II collagen as well as a lower deposition of type I collagen and also better surface integrity and a significant increase in the number of chondrocytes.CONCLUSION:
LP-PRP treatment favored restoration in early osteoarthritic cartilage and reduced the pro-inflammatory effect of IL-1ß. LP-PRP is a promising therapy for early osteoarthritis, as it promotes extracellular matrix repair, reduces inflammation, and slows cartilage degeneration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoartrite
/
Cartilagem Articular
/
Plasma Rico em Plaquetas
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article