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Neuroprotective effects of quercetin 4'-O-ß-d-diglucoside on human striatal precursor cells in nutrient deprivation condition.
Sarchielli, Erica; Morelli, Annamaria; Guarnieri, Giulia; Iorizzi, Maria; Sgambati, Eleonora.
Afiliação
  • Sarchielli E; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy.
  • Morelli A; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy.
  • Guarnieri G; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy.
  • Iorizzi M; Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090, Pesche, Isernia, Italy.
  • Sgambati E; Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090, Pesche, Isernia, Italy. Electronic address: eleonora.sgambati@unimol.it.
Acta Histochem ; 120(2): 122-128, 2018 Feb.
Article em En | MEDLINE | ID: mdl-29336843
Several investigations have demonstrated neuroprotective effects of quercetin, a polyphenol widely present in nature, against neurotoxic chemicals, as well as in neuronal injury/neurodegenerative disease models. Most of these studies have been performed with quercetin aglycone and its metabolites, while scanty data are available on its glycosides. This study is aimed at investigating the neuroprotective effects of quercetin 3,4'-O-ß-d-diglucoside (Q3,4'dG), isolated from the bulbs of the white cultivar (Allium cepa L.), using an in vitro model of human striatal precursor cells (HSPs), a primary culture isolated from the striatal primordium and previously characterized. To study the effect of Q3,4'dG on cell survival, HSPs were exposed to nutrient deprivation created by replacing culture medium with phosphate buffer saline (PBS). Our findings showed that Q3,4'dG treatment significantly promoted cell survival and strongly decreased apoptosis induced by nutrient deprivation, as evaluated by cell proliferation/death analyses. In addition, since the adhesive capacities of cells are essential for cell survival, the expression of some adhesion molecules, such as pancadherin and focal adhesion kinase, was evaluated. Interestingly, PBS exposure significantly decreased the expression of both molecules, while in the presence of Q3,4'dG this effect was prevented. This study provides evidence of a neuroprotective role exerted by Q3,4'dG and suggests its possible implication in sustaining neuronal survival for prevention and treatment of neurodegenerative disorders.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Fármacos Neuroprotetores / Corpo Estriado / Células Precursoras de Oligodendrócitos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Fármacos Neuroprotetores / Corpo Estriado / Células Precursoras de Oligodendrócitos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article