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A hybrid protein containing MSP1a repeats and Omp7, Omp8 and Omp9 epitopes protect immunized BALB/c mice against anaplasmosis.
Cangussu, Alex Sander R; Mariúba, Luis André M; Lalwani, Pritesh; Pereira, Keila Dayane E S; Astolphi-Filho, Spartaco; Orlandi, Patricia P; Epiphanio, Sabrina; Viana, Kelvison F; Ribeiro, Mucio Flavio B; Silva, Hidelberto M; Marinho, Claudio R F; Nogueira, Paulo A.
Afiliação
  • Cangussu ASR; Universidade Federal do Amazonas-Programa de pós-graduação em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Manaus, AM/Brasil, Brazil. alexcangussu@uft.edu.br.
  • Mariúba LAM; Universidade Federal do Tocantins-Engenharia de Bioprocessos e Biotecnologia, Gurupi, TO/Brazil, Brazil. alexcangussu@uft.edu.br.
  • Lalwani P; Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Manaus, Amazonas/Brazil, Brazil.
  • Pereira KDES; Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Manaus, Amazonas/Brazil, Brazil.
  • Astolphi-Filho S; Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Manaus, Amazonas/Brazil, Brazil.
  • Orlandi PP; Universidade Federal do Amazonas-Programa de pós-graduação em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Manaus, AM/Brasil, Brazil.
  • Epiphanio S; Instituto Leônidas e Maria Deane-Fiocruz Amazônia, Manaus, Amazonas/Brazil, Brazil.
  • Viana KF; Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, São Paulo/Brazil, Brazil.
  • Ribeiro MFB; Universidade Federal do Tocantins-Engenharia de Bioprocessos e Biotecnologia, Gurupi, TO/Brazil, Brazil.
  • Silva HM; Departamento de Parasitologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais/Brazil, Brazil.
  • Marinho CRF; Faculdade de Medicina, Centro Universitário Unirg, Gurupi, Tocantins/Brazil, Brazil.
  • Nogueira PA; Departamento de Parasitologia do Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Vet Res ; 49(1): 6, 2018 01 19.
Article em En | MEDLINE | ID: mdl-29351812
Anaplasma marginale (A. marginale) has a remarkable impact on livestock production, and an effective vaccine is not currently available due to the inexistence of a small animal model. Recently, BALB/c mice were successfully infected with A. marginale, resulting in an acute and persistent anaplasmosis infection. Here, we designed a hybrid protein containing repeats of polypeptide 1a from major surface protein-1 complex (MSP1a) repeats and common epitopes of outer membrane proteins (OMPs) OMP7, OMP8 and OMP9 expressed in Escherichia coli. Our proof-of-concept assessed vaccinal effectiveness against a challenge with live bacteria. The MSP1a/OMP7/8/9 immunized BALB/C mice exhibited a strong reduction in rickettsemia and had no signs of anaplasmosis or hepatic lesions. In contrast, the non-immunized mice exhibited signs of anaplasmosis and a body weight loss associated with increases in monocyte and neutrophil counts. Furthermore, the non-immunized mice displayed atrophies with chronic inflammatory infiltrates in the spleen and increased binucleation and hydropic degeneration in the hepatocytes. Our findings demonstrated that immunization with our hybrid protein induced a strong reduction in rickettsemia and conferred protection against anaplasmosis. Therefore, given the strong evidence of the protective effect against anaplasmosis, hybrid protein designs are potential candidates for the rational design of vaccinal subunits.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Anaplasmose / Epitopos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas da Membrana Bacteriana Externa / Anaplasmose / Epitopos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article