Diagnostic value of human papillomavirus (HPV) 16 and HPV18 viral loads for the detection of high-grade cervical intraepithelial neoplasia (CIN2+) in a cohort of African women living with HIV.
J Clin Virol
; 99-100: 79-83, 2018.
Article
em En
| MEDLINE
| ID: mdl-29353074
ABSTRACT
BACKGROUND:
African women living with HIV (WLHIV) are at high risk of cervical cancer but rarely adequately screened. Better strategies enabling identification of WLHIV with high-grade cervical intraepithelial lesions (CIN2+) are required.OBJECTIVES:
To investigate the diagnostic value of HPV16 and HPV18 viral loads in a cohort of African WLHIV.DESIGN:
HPV16 and HPV18 viral loads were determined by quantitation of the E6 gene DNA by real-time PCR in cervical specimens collected at baseline and endline (16 months) from 245 African WLHIV positive for HPV16 or/and HPV18. Cervical biopsies were graded using the histopathological CIN classification.RESULTS:
Women with CIN2+ had higher viral load for HPV16 (pâ¯<â¯0.0001) or HPV18 (pâ¯=â¯0.03) than those without CIN2+. HPV16 viral load ≥3.59 log copies/1000 cells detected CIN2+ with sensitivity and specificity of 93.5% (95%CI 81.7-98.3%) and 74.1% (95%CI 66.3-80.6%), respectively, whereas HPV18 viral load ≥1.63 log copies/1000 cells detected CIN2+ with sensitivity and specificity of 59.1% (95%CI 38.7-76.7%) and 66.9% (95%CI 58.8-74.1%), respectively. A high baseline HPV16 viral load was significantly associated with persistence of, or progression to CIN2+ at endline; these findings were not observed for HPV18.CONCLUSIONS:
HPV16 viral load is a powerful marker of CIN2+ in African WLHIV. HPV18 viral load is of lower diagnostic value in this population.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Displasia do Colo do Útero
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Neoplasias do Colo do Útero
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Carga Viral
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Infecções por Papillomavirus
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Papillomavirus Humano 16
/
Papillomavirus Humano 18
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Limite:
Adolescent
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Adult
/
Female
/
Humans
/
Middle aged
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article