DExSI: a new tool for the rapid quantitation of 13C-labelled metabolites detected by GC-MS.
Bioinformatics
; 34(11): 1957-1958, 2018 06 01.
Article
em En
| MEDLINE
| ID: mdl-29360933
ABSTRACT
Summary:
Stable isotope directed metabolomics is increasingly being used to measure metabolic fluxes in microbial, plant and animal cells. Incorporation of 13C/15N isotopes into a wide range of metabolites is typically determined using gas chromatography-mass spectrometry (GC/MS) or other hyphenated mass spectrometry approaches. The DExSI (Data Extraction for Stable Isotope-labelled metabolites) pipeline is an interactive graphical software package which can be used to rapidly quantitate isotopologues for a wide variety of metabolites detected by GC/MS. DExSI performs automated metabolite annotation, mass and positional isotopomer abundance determination and natural isotope abundance correction. It provides a range of output options and is suitable for high throughput analyses. Availability and implementation DExSI is available for non-commercial use from https//github.com/DExSI/DExSI/. For Microsoft Windows 7 or higher (64-bit). Contact malcolmm@unimelb.edu.au or michael.dagley@unimelb.edu.au. Supplementary information Supplementary data are available at Bioinformatics online.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Isótopos de Carbono
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Metabolômica
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Marcação por Isótopo
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Cromatografia Gasosa-Espectrometria de Massas
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article