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Circular RNA-ZFR Inhibited Cell Proliferation and Promoted Apoptosis in Gastric Cancer by Sponging miR-130a/miR-107 and Modulating PTEN.
Liu, Tonglei; Liu, Shuang; Xu, Yu; Shu, Ruo; Wang, Feng; Chen, Cheng; Zeng, Yujian; Luo, Huayou.
Afiliação
  • Liu T; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Liu S; Department of Ultrasonics, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Xu Y; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Shu R; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Wang F; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Chen C; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Zeng Y; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Luo H; Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Cancer Res Treat ; 50(4): 1396-1417, 2018 Oct.
Article em En | MEDLINE | ID: mdl-29361817
PURPOSE: This study aimed to probe into the associations among circular RNA ZFR (circ-ZFR), miR-130a/miR-107, and PTEN, and to investigate the regulatory mechanism of circ-ZFR‒miR-130a/miR-107‒PTEN axis in gastric cancer (GC). MATERIALS AND METHODS: GSE89143 microarray data used in the study were acquired from publicly available Gene Expression Omnibus database to identify differentially expressed circular RNAs inGC tissues. The expressions of circ-ZFR, miR-130a, miR-107, and PTEN were examined by real-time reverse transcription polymerase chain reaction, while PTEN protein expression was measured by western blot. The variation of GC cell proliferation and apoptosis was confirmed by cell counting kit-8 assay and flow cytometry analysis. The targeted relationships among circZFR, miR-130a/miR-107, and PTEN were predicted via bioinformatics analysis and demonstrated by dual-luciferase reporter assay and RNA immunoprecipitation assay. The impact of ZFR on gastric tumor was further verified in xenograft mice model experiment. RESULTS: Circ-ZFR and PTEN were low-expressed whereas miR-107 and miR-130a were highexpressed in GC tissues and cells. There existed targeted relationships and interactions between miR-130a/miR-107 and ZFR/PTEN. Circ-ZFR inhibited GC cell propagation, cell cycle and promoted apoptosis by sponging miR-107/miR-130a, while miR-107/miR-130a promoted GC cell propagation and impeded apoptosis through targeting PTEN. Circ-ZFR inhibited cell proliferation and facilitated apoptosis in GC by sponging miR-130a/miR-107 and modulating PTEN. Circ-ZFR curbed GC tumor growth and affected p53 protein expression in vivo. CONCLUSION: Circ-ZFR restrained GC cell proliferation, induced cell cycle arrest and promoted apoptosis by sponging miR-130a/miR-107 and regulating PTEN.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / RNA / MicroRNAs / PTEN Fosfo-Hidrolase Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / RNA / MicroRNAs / PTEN Fosfo-Hidrolase Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article