A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro.
J Vis Exp
; (131)2018 01 09.
Article
em En
| MEDLINE
| ID: mdl-29364233
ABSTRACT
Natural killer (NK) cells belong to the innate immune system and are a first-line anti-cancer immune defense; however, they are suppressed in the tumor microenvironment and the underlying mechanism is still largely unknown. The lack of a consistent and reliable source of NK cells limits the research progress of NK cell immunity. Here, we report an in vitro system that can provide high quality and quantity of bone marrow-derived murine NK cells under a feeder-free condition. More importantly, we also demonstrate that siRNA-mediated gene silencing successfully inhibits the E4bp4-dependent NK cell maturation by using this system. Thus, this novel in vitro NK cell differentiating system is a biomaterial solution for immunity research.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Matadoras Naturais
/
Técnicas Citológicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article