Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics.
Nat Methods
; 15(3): 187-190, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-29377012
ABSTRACT
For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here we report a method revealing widespread and abundant protein histidine phosphorylation in Escherichia coli. We generated an extensive E. coli phosphoproteome data set, in which a remarkably high percentage (â¼10%) of phosphorylation sites are phosphohistidine sites. This resource should help enable a better understanding of the biological function of histidine phosphorylation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Proteínas de Escherichia coli
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Proteômica
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Escherichia coli
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Histidina
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article