[The investigation the combined effect of SNP rs9939609 (gene FTO) and rs4994 (gene ADRB3) polymorphisms on risk of obesity].

ABSTRACT

The genetic factor plays a significant role in the development of obesity, by present time the association of hundreds genetic polymorphisms with the risk of this disease is established. However, the combined influence of genetic polymorphisms remains practically unstudied. We aimed to investigate the combined effect SNP rs9939609 (gene FTO) and rs4994 (gene ADRB3) polymorphisms on risk of obesity. A case-control study was conducted, including255 obese case (BMI>30 kg/m2) and 427 non obese controls (BMI<30 kg/m2). Genotyping was performed using allele-specific amplification, detection results in real time using TaqMan-probes complementary DNA polymorphic sites. It has been shown, that presence of one mutant allele of rs9939609 (gene FTO) and rs4994 (gene ADRB3) leads to statistically significant association with obesity. Presence of two mutant alleles in different polymorphic variants increases risk of obesity by 15%, presence of three mutant alleles - by 2.63 fold. The quantity surveyed, suffering obesity, increased depending on the number of mutant alleles in studied genetic polymorphisms. Presence of one or two mutant alleles in one polymorphic variant increased the number of patients with obesity by 13.4%, presence of two or three mutant alleles in different polymorphic variants -by 18-19%.