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Cellular distribution of secretory pathway markers in the haploid synergid cells of Arabidopsis thaliana.
Jones, Daniel S; Liu, Xunliang; Willoughby, Andrew C; Smith, Benjamin E; Palanivelu, Ravishankar; Kessler, Sharon A.
Afiliação
  • Jones DS; Department of Microbiology and Plant Biology, The University of Oklahoma, Norman, OK, 73069, USA.
  • Liu X; School of Plant Sciences, University of Arizona, Tucson, Arizona, 85721, USA.
  • Willoughby AC; Department of Microbiology and Plant Biology, The University of Oklahoma, Norman, OK, 73069, USA.
  • Smith BE; Vision Sciences, University of California, Berkeley, CA, 94720, USA.
  • Palanivelu R; School of Plant Sciences, University of Arizona, Tucson, Arizona, 85721, USA.
  • Kessler SA; Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.
Plant J ; 94(1): 192-202, 2018 04.
Article em En | MEDLINE | ID: mdl-29385641
ABSTRACT
In flowering plants, cell-cell communication plays a key role in reproductive success, as both pollination and fertilization require pathways that regulate interactions between many different cell types. Some of the most critical of these interactions are those between the pollen tube (PT) and the embryo sac, which ensure the delivery of sperm cells required for double fertilization. Synergid cells function to attract the PT through secretion of small peptides and in PT reception via membrane-bound proteins associated with the endomembrane system and the cell surface. While many synergid-expressed components regulating PT attraction and reception have been identified, few tools exist to study the localization of membrane-bound proteins and the components of the endomembrane system in this cell type. In this study, we describe the localization and distribution of seven fluorescent markers that labelled components of the secretory pathway in synergid cells of Arabidopsis thaliana. These markers were used in co-localization experiments to investigate the subcellular distribution of the two PT reception components LORELEI, a GPI-anchored surface protein, and NORTIA, a MILDEW RESISTANCE LOCUS O protein, both found within the endomembrane system of the synergid cell. These secretory markers are useful tools for both reproductive and cell biologists, enabling the analysis of membrane-associated trafficking within a haploid cell actively involved in polar transport.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Via Secretória Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Via Secretória Idioma: En Ano de publicação: 2018 Tipo de documento: Article