Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration.
Eur J Immunol
; 48(6): 1074-1077, 2018 06.
Article
em En
| MEDLINE
| ID: mdl-29436709
ABSTRACT
Estrogen inducible Hoxb8 leads to conditional immortalization of hematopoietic precursors. These cells can be cultured and infected with the CRISPR/Cas9 system for genome editing, circumventing resource consuming generation of mouse models. The resultant cells retain their ability to differentiate into migratory dendritic cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Células-Tronco Hematopoéticas
/
Engenharia Genética
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Movimento Celular
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Proteínas de Homeodomínio
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article