Human Vδ3+ γδ T cells induce maturation and IgM secretion by B cells.
Immunol Lett
; 196: 126-134, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29438730
ABSTRACT
This study tested the hypothesis that the Vδ3 subset of human γδ T cells, like their Vδ2 counterparts, can influence differentiation, antibody secretion and cytokine production by B cells. Vδ3â¯T cells constitute a minor subset of peripheral blood lymphocytes but are enriched in the liver and gut and are expanded in patients with cytomegalovirus activation and B cell chronic lymphocytic leukemia. They have been reported to include MHC class I and CD1d restricted cells. Like Vδ2â¯T cells, they are capable of maturing dendritic cells into cytokine-producing antigen presenting cells, making them potential targets for dendritic cell-based immunotherapies. Since it is unknown if Vδ3â¯T cells can also provide B cell help, we investigated if Vδ3â¯T cells can promote B cell differentiation, antibody secretion and cytokine production in vitro. Vδ3â¯T cells were sorted from healthy human blood and expanded using phytohemagglutinin and cultured with freshly isolated human B cells. We found that Vδ3â¯T cells and B cells reciprocally induced expression of maturation markers CD40, CD86 and HLA-DR but not TH1, TH2 or TH17 cytokines. Furthermore, Vδ3â¯T cells promoted the release of IgM, but not IgG, IgA or IgE by B cells. These data demonstrate, for the first time, a reciprocal activating relationship between Vδ3â¯T cells and B cells, which could prove a useful target for cellular immunotherapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina M
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Linfócitos B
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Linfócitos T
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Receptores de Antígenos de Linfócitos T gama-delta
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article