Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity.

Prajapati, Anil S; Pawar, Vishakha A; Panchal, Ketankumar J; Sudhir, Ankit P; Dave, Bhaumik R; Patel, Darshan H; Subramanian, R B

Prajapati, Anil S; Pawar, Vishakha A; Panchal, Ketankumar J; Sudhir, Ankit P; Dave, Bhaumik R; Patel, Darshan H; Subramanian, R B.

Afiliação

Prajapati AS; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
Pawar VA; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
Panchal KJ; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
Sudhir AP; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
Dave BR; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
Patel DH; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology (CHARUSAT), Changa, Anand, Gujarat, India.
Subramanian RB; P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India. promat103vans@gmail.com.

BMC Biotechnol ; 18(1): 9, 2018 02 13.

Article em En | MEDLINE | ID: mdl-29439688

Assuntos

Bacillus amyloliquefaciens/enzimologia; Endo-1,4-beta-Xilanases/genética; Endo-1,4-beta-Xilanases/metabolismo; Substituição de Aminoácidos; Bacillus amyloliquefaciens/genética; Sítios de Ligação; Celulose/metabolismo; Clonagem Molecular; Detergentes/química; Endo-1,4-beta-Xilanases/química; Endo-1,4-beta-Xilanases/isolamento & purificação; Estabilidade Enzimática; Concentração de Íons de Hidrogênio; Cinética; Metais/química; Mutagênese Sítio-Dirigida; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Especificidade por Substrato; Xilanos/metabolismo

Palavras-chave

Protein engineering; Site-directed mutagenesis; Substrate specificity; Xylanase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endo-1,4-beta-Xilanases / Bacillus amyloliquefaciens Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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