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Transcriptome dynamics at Arabidopsis graft junctions reveal an intertissue recognition mechanism that activates vascular regeneration.
Melnyk, Charles W; Gabel, Alexander; Hardcastle, Thomas J; Robinson, Sarah; Miyashima, Shunsuke; Grosse, Ivo; Meyerowitz, Elliot M.
Afiliação
  • Melnyk CW; Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom; charles.melnyk@slu.se.
  • Gabel A; Department of Plant Biology, Swedish University of Agricultural Sciences, 756 51 Uppsala, Sweden.
  • Hardcastle TJ; Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom.
  • Robinson S; Institute of Computer Science, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
  • Miyashima S; Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.
  • Grosse I; Institute of Plant Science, University of Bern, 3013 Bern, Switzerland.
  • Meyerowitz EM; Graduate School of Biological Sciences, Nara Institute of Science and Technology, 630-0192 Ikoma, Japan.
Proc Natl Acad Sci U S A ; 115(10): E2447-E2456, 2018 03 06.
Article em En | MEDLINE | ID: mdl-29440499
ABSTRACT
The ability for cut tissues to join and form a chimeric organism is a remarkable property of many plants; however, grafting is poorly characterized at the molecular level. To better understand this process, we monitored genome-wide gene expression changes in grafted Arabidopsis thaliana hypocotyls. We observed a sequential activation of genes associated with cambium, phloem, and xylem formation. Tissues above and below the graft rapidly developed an asymmetry such that many genes were more highly expressed on one side than on the other. This asymmetry correlated with sugar-responsive genes, and we observed an accumulation of starch above the graft junction. This accumulation decreased along with asymmetry once the sugar-transporting vascular tissues reconnected. Despite the initial starvation response below the graft, many genes associated with vascular formation were rapidly activated in grafted tissues but not in cut and separated tissues, indicating that a recognition mechanism was activated independently of functional vascular connections. Auxin, which is transported cell to cell, had a rapidly elevated response that was symmetric, suggesting that auxin was perceived by the root within hours of tissue attachment to activate the vascular regeneration process. A subset of genes was expressed only in grafted tissues, indicating that wound healing proceeded via different mechanisms depending on the presence or absence of adjoining tissues. Such a recognition process could have broader relevance for tissue regeneration, intertissue communication, and tissue fusion events.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis / Feixe Vascular de Plantas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis / Feixe Vascular de Plantas Idioma: En Ano de publicação: 2018 Tipo de documento: Article