Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.

Leitão, Elsa; Beygo, Jasmin; Zeschnigk, Michael; Klein-Hitpass, Ludger; Bargull, Marcel; Rahmann, Sven; Horsthemke, Bernhard

Leitão, Elsa; Beygo, Jasmin; Zeschnigk, Michael; Klein-Hitpass, Ludger; Bargull, Marcel; Rahmann, Sven; Horsthemke, Bernhard.

Afiliação

Leitão E; Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Beygo J; Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Zeschnigk M; Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Klein-Hitpass L; Institute of Cell Biology, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Bargull M; Genome Informatics, Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Rahmann S; Genome Informatics, Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany.
Horsthemke B; Institute of Human Genetics, University of Duisburg-Essen, University Hospital Essen, Essen, Germany. bernhard.horsthemke@uni-due.de.

Methods Mol Biol ; 1767: 351-366, 2018.

Article em En | MEDLINE | ID: mdl-29524145

Assuntos

Metilação de DNA; DNA/genética; Sequenciamento de Nucleotídeos em Larga Escala/métodos; Animais; Sequência de Bases; Ilhas de CpG; Primers do DNA/genética; Humanos; Camundongos; Reação em Cadeia da Polimerase/métodos; Software; Sulfitos/química

Palavras-chave

Amplicon bisulfite sequencing; Amplikyzer software; DNA methylation; MiSeq system

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Metilação de DNA / Sequenciamento de Nucleotídeos em Larga Escala Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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