Isolation of Schwann Cell Precursors from Rodents.
Methods Mol Biol
; 1739: 3-15, 2018.
Article
em En
| MEDLINE
| ID: mdl-29546697
ABSTRACT
Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically ß-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células de Schwann
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article