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High expression of glutamate-ammonia ligase is associated with unfavorable prognosis in patients with ovarian cancer.
Fan, Shaohua; Wang, Yanyan; Zhang, Zifeng; Lu, Jun; Wu, Zhiyong; Shan, Qun; Sun, Chunhui; Wu, Dongmei; Li, Mengqiu; Sheng, Ning; Xie, Ying; Zheng, Yuanlin.
Afiliação
  • Fan S; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Wang Y; Department of Medical Ultrasonics, The Affiliated First People's Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  • Zhang Z; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Lu J; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Wu Z; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, P.R. China.
  • Shan Q; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Sun C; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Wu D; Department of Medical Ultrasonics, The Affiliated First People's Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  • Li M; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Sheng N; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Xie Y; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
  • Zheng Y; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P.R. China.
J Cell Biochem ; 119(7): 6008-6015, 2018 07.
Article em En | MEDLINE | ID: mdl-29575012
Glutamate-ammonia ligase (GLUL), which is also called GS (glutamine synthetase), is the enzyme that catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. Here, we found higher expression of GLUL in the ovarian cancer patients was associated with worse disease-free survival (DFS) and overall survival (OS). In addition, GLUL was heterogeneously expressed in various ovarian cancer cells. The mRNA and protein expression levels of GLUL in NIH:OVCAR-3 and ES-2 cells were obviously higher than that in the other types of ovarian cancer cells. Knockdown of GLUL in NIH:OVCAR-3 or ES-2 cells could significantly decrease the proliferation ability. Furthermore, GLUL knockdown markedly inhibited the p38 MAPK signaling pathway in NIH:OVCAR-3 or ES-2 cells. Our findings suggest that decreasing expression of GLUL may be a new approach that can be used for ovarian cancer treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Glutamato-Amônia Ligase Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Glutamato-Amônia Ligase Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article